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Vector Laboratories vector red substrate kits sk 5100
Vector Red Substrate Kits Sk 5100, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Red Alkaline Phosphatase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories cd31 staining
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Cd31 Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectorred substrate kit
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Vectorred Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vector red alkaline phosphatase kit
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Vector Red Alkaline Phosphatase Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories diaminobenzidine substrate kit
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Diaminobenzidine Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Enzo Biochem vectorv r red-kit alkaline phosphatase substrate sk-5100
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Vectorv R Red Kit Alkaline Phosphatase Substrate Sk 5100, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectashield antifade mounting medium
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Vectashield Antifade Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories ap substrate kit
Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and <t>anti-CD31</t> (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.
Ap Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and anti-CD31 (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.

Journal: Journal of Tissue Engineering

Article Title: Toward 3D-bioprinting of an endocrine pancreas: A building-block concept for bioartificial insulin-secreting tissue

doi: 10.1177/20417314221091033

Figure Lengend Snippet: Chorioallantoic membrane assay is a suitable model for investigating angiogenesis in tissue-engineered grafts. Extensive, rapid vascular ingrowth is seen in both PCL and cell-laden hydrogel structure after the 9-day assay period. Ex ovo CAM assay experiments enabled direct comparison of heparinized PCL scaffolds with untreated controls and validated the beneficial properties of heparinization for enhanced vascular ingrowth ((a–c) arrow indicates eye of chicken embryo). In ovo CAM assay experiments were used for investigation of 3D-bioprinted INS-1-laden droplets ((d–l) arrow indicates xenotransplant). Vascular structures (arrows) penetrated into the scaffold (g–l). (g) Anti-insulin immunohistochemical staining of CAM assay explant. (h) H&E staining of CAM assay explant. (i–l) Anti-insulin (brown) and anti-CD31 (red) immunohistochemical double-staining of CAM assay explant. Rapid vascularization maintained viability and function of pseudoislets. Peri- and intra-insular (asterisks) vessels were detected (g–l). Scale bar (a and d) 10 mm, (b, c, e, and f) 2 mm, (j) 100 µm, (g, h, k, and l) 50 µm, (i) 20 µm.

Article Snippet: For final validation of spatial relationships, tissue slices were double-stained using both a primary insulin (2D11-H5, Santa Cruz) and CD-31 antibodies (overnight 1:250 in background reducing agent, monoclonal rabbit IgG; Lot# EPR17259, ab182981, Abcam, Cambridge, UK ) using 3-3′diaminobenzidine staining for insulin, a biotin-streptavidin system for CD31 staining (Vector® BA-1000, goat anti-rabbit secondary IgG; Lot# ZH0818; IgG, and Vector® Red Substrate Kit SK-5100, Lot# Reagent1 ZH0305, Reagent2 ZH0308, Reagent3 ZH0305 Vector Laboratories Inc., Burlingame, CA, USA) and subsequent hematoxylin counter-staining.

Techniques: Chick Chorioallantoic Membrane Assay, In Ovo, Immunohistochemical staining, Staining, Double Staining